FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1982_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1982_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGCACTGTGGGTATGCTGGCTGCACGCCCAGCGATGTGTGATTCTG50.17018379850238255No Hit
GCTCTGTGTTAGGTGCTGTGGGCAATCCAAGGGGCCTTCGACACCACCTT40.13614703880190604No Hit
GTGTAAGTGGGATTTAGCCTCAACTAGACTAGTTCAGGCTGTGAACAGGG40.13614703880190604No Hit
CCCCTCACCCGTCCCCGGCTCCTGACATCCTTGCTTCCAGCCAGGTCGCT40.13614703880190604No Hit
GAGCAGGGACTTAAGCCTCCTCCAGCTGATGAAGTATGAAAGGGGGTGAC30.10211027910142954No Hit
GTGTGATGGGAGACCTAAGACATGGCCCACAGGTGCACCTGTGTTGAAAG30.10211027910142954No Hit
CTCCTGCAGAGATCAGAGAGCCAGAGCTCACTCCGTCCTCTGTCTTGCAA30.10211027910142954No Hit
ATCTGAGGCCGAGAGGCCAGCTCTGTTTGTGCCAGTCTGTCCCCAGGTGT30.10211027910142954No Hit
CTCCATGTCTGTTCATTAGTCACTTCTCCCTGCAATGGGGAAAAAAAAGT30.10211027910142954No Hit
GGGCAGGACTGTCCCTGGAAGTGCGTGACTTGGTGCACGAACAGGCTTGT30.10211027910142954No Hit
TCTCTCTGCTGCACCAAAAATCCCACAACTAATTACATGACCATCATCGT30.10211027910142954No Hit
GCTCTGTACCCATCAGATCGCAGCCCTGATCTCTCCTGCTGATGCACACA30.10211027910142954No Hit
CTCCTGTACCGCCCAGGATGCAGAGAGGGGCGACAACAATCATGCTGGGC30.10211027910142954No Hit
CCCCTACACTAAGCATGATGTTAAATCTGTCAATAGAGGAAGGCCATTGA30.10211027910142954No Hit
GCTGTAACTGATTTGCATCCCAGCCTGGACAGCGGACGTACCTGGCACTT30.10211027910142954No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG30.10211027910142954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGAC100.006732901145.07
AAAAAAA400.00755564218.12550-54
Produced by FastQC (version 0.11.5)