FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1982_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1982_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2409
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCATATCCTTCATGTACTTTTATCTTCAATGTTTGGAGGTTGCCTGTA50.20755500207555005No Hit
CTATCAGGATTATGTAAATGTTATTCAGTTCTGTAATACAGCATGCCATA50.20755500207555005No Hit
GTAATACAGCATGCCATAATTAAACCTTTTCTTTTCCTGTACAACTTTTC40.16604400166044No Hit
AATCAGCACTGTGTCCTTGCAGACTTAATTAGCAGCTCTAGTTGAAACTC40.16604400166044No Hit
ACATGGGGCTCAGTCCCAGGACACGTGCATGATGACCTGAGCCAAAGGTT40.16604400166044No Hit
ATTCTGGCCCGTTAATCTCCCACATCATAGACGATGAATAAGTTATCGTC30.12453300124533001No Hit
GTGTGGGAGGAAGGAAACAAATCAGGCTCCACTTCAATGAGGGGGGACTT30.12453300124533001No Hit
GTCTACAAAGGATGGACTTGAGTTTTCACGTCGACAGGCTGACCGTGTGC30.12453300124533001No Hit
GTGCATGCATGGACTCCCCCCGCCAACAATCGCTGTCCCAGCTCTGCACC30.12453300124533001No Hit
CTTCTATATATACGCTGCTTGTCCCGCCAGCCACGGGTAGGAATCTGGTC30.12453300124533001No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA30.12453300124533001No Hit
GCCTTATATACTTGAAAGTTACTCGGAAACTAGATCTTAAATGTTCTCAC30.12453300124533001No Hit
CTATGGCACATTTCCAGGAGTGAAGCCAATGGGTCTGCTGATACTGTTGC30.12453300124533001No Hit
GTCTTATGGGAAATGGAGTCTCTCTCTCCTCCTCTGGACTTCTTCATATT30.12453300124533001No Hit
CCTCCGTACCTGGCAAAAATCACTGCAAAAATACTTCCCTAAAAGAAAGA30.12453300124533001No Hit
ATCAATACATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGAT30.12453300124533001No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG30.12453300124533001No Hit
GATGTACACACCAGGAGGGGAGGTAAGAAATGATGATGTTGACCAAAGTT30.12453300124533001No Hit
GGTATGGGGAAGGTGTGTGGCTTCTGTGCCCAACACAAATGTTCTTTTTT30.12453300124533001No Hit
CCACAGAGGTGATACACAAATTCAAACTAGAAGATCATTTGAATTGAAGA30.12453300124533001No Hit
GACTCAGAGGTTTAAGCAGCAAAGCTATTTAGGTCTCACTGTGAGAATTA30.12453300124533001No Hit
ATTAAGAAGTACACATCAGGAAGACAGGAGAAGAATCCCGCTCTTAGAAT30.12453300124533001No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG30.12453300124533001No Hit
GGTTTAAGCAGCAAAGCTATTTAGGTCTCACTGTGAGAATTATTTTGAGC30.12453300124533001No Hit
CATTAGCTTTCTCCCCTTTCGTAAGATTGCTCAGTTCATTGATGCTTAAT30.12453300124533001No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAAGTA100.0066591203145.05
ATATAGA100.0066591203145.01
AAAAAAA600.004352953814.50000185-89
Produced by FastQC (version 0.11.5)