FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1982-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1982-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169717
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG1352.141996E-532.2233545
TCGACAC1504.399567E-528.999777145
AACGTTG2151.8655814E-523.6054785
TATAGTA3905.020411E-1022.3132442
TATACTG5450.021.285155
CTATAGT6150.021.2284241
CCTAGAC7100.020.4276183
AGACTAC5052.910383E-1120.0988566
TACTATA2556.9454676E-519.9069142
GTATAAT8400.018.9960691
GTATAAG4603.912646E-918.9209861
GTATTAA13900.018.7848641
TATGATG9750.018.5903955
TATAATA7600.018.129512
CTGATCG2801.426427E-418.124869
GTATTAT12100.017.9827541
TCCTATG10100.017.950012
ATTATAC5751.8371793E-1017.6565673
TAGGAGT5351.3351382E-917.6189464
GTATAAC4608.054485E-817.3442361
Produced by FastQC (version 0.11.5)