FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1982-d10-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1982-d10-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628982
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2600.041.8302271
ATACTAC2450.038.4693683
GCGAAAG2950.036.8643882
TACTACA2950.036.8643884
TGTATAA2151.8082574E-830.3488222
GTCCTAT4050.028.6442381
CCTATAT1555.4942662E-528.0667321
GTGTATA4150.027.9540141
ACCTATT1855.811662E-627.4324172
CGAAAGC4000.027.1874853
TATACTA4000.027.1874852
CGGCCGA1100.00966893526.3636239
CTATGAT3950.025.6961884
TAGACTG2651.3746285E-724.622635
TTAGACT3004.5494016E-721.749994
CATATAC2003.1012794E-421.7499894
GTTATAG2404.3569227E-521.1475031
CTATGAC3551.0132317E-720.4225233
TTATATC3208.4604653E-720.3906143
AACTATG2155.0532864E-420.2325487
Produced by FastQC (version 0.11.5)