FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1982-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1982-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences987196
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATGCTGCCCACTTAACAACATAAACCCAACCGGCTGATCCCGTTC14960.1515403222865571No Hit
GCATATGGATGCGCTGTTTATCGATCCTGATGTGCGCGGCTGCGGCGTGG11830.1198343591343563No Hit
GATTTAATCTGTATCAGGCTGAAAATCTTCTCTCAATCCGCCAAAACAGC10140.1027151649723054No Hit
GGTCATAGTGATCCGGTGGTTCTGAATGGAAGGGCCATCGCTCAACGGAT10070.10200608592417311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATAGT4700.040.1063273
TCGAAAC2950.036.8643539
CCTATAG3050.035.655692
GTCATAG5000.034.799952
GTCTAAT2800.033.6674921
TGATCCG5000.033.3499539
TATACTG2008.951247E-932.6249545
GCATCGA5250.031.761862145
TAATACG1402.746666E-531.0713864
GTATAAA6100.029.7190951
TATAGTC3750.028.9999624
CTATAGT3800.028.618383
ATAGTCG3850.028.2467145
CCCTATA4150.027.9574321
CGAAAGC7550.027.8476433
AGTCGAA3950.027.5316077
CTATAGG2954.820322E-1027.033862
TATAGGG3005.875336E-1026.5832963
AGTGATC7100.026.549267
CCATATG10750.026.302292
Produced by FastQC (version 0.11.5)