FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1982-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1982-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658456
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGTG1209.559268E-636.249797
TAGACGT1051.8530933E-434.523614
AGCGAAA2653.6379788E-1232.8499531
GAACCGA1804.6969744E-628.194286
GCGAAAG3102.7284841E-1128.0686172
CCTATAT3005.820766E-1026.599341
ACATCCG1100.00966950426.3634855
CAGATCG1658.414615E-526.3634839
CGAAAGC3408.913048E-1125.590033
ATTATAC2051.28869215E-524.7578353
CTATAAG3851.6370905E-1124.495261
GTCCTAT5450.023.959371
GATCGCT551.9715844E-723.72713510-14
ATACACG2151.8634693E-523.6063083
GGTATAC1902.1831458E-422.9085221
GGTATAA4750.022.9085221
TATAGAC1902.1898959E-422.898082
TATTATA3203.3758624E-822.6595612
ATATATC2252.6483283E-522.557143
GTCTAAT1952.6028053E-422.3211251
Produced by FastQC (version 0.11.5)