FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1982-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1982-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550461
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9670.17567093763227548No Hit
CTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTCATT5670.10300457253102399No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCA400.00604955154.374765
GTGATCG657.333857E-444.615199
TATACTG1854.1745807E-935.2701155
TTATACT1352.1405274E-532.222084
CCTATAC1153.1603474E-431.52163
GCGAAAG3300.030.7602372
AGCGAAA3400.029.8690871
GCTATAT2003.2344906E-729.0156861
GTATAGG1000.00605609329.0156861
CGAAAGC3500.028.9998743
ATTAGAC2054.0337363E-728.292563
GTACCGC1050.00770565427.6189276
GTATAAT1855.7855286E-627.4472731
GTATATA3750.027.0813061
TATTAGG1100.00966380726.3659172
CTATATA1958.739284E-626.0278912
GTCCTAT6700.025.9841961
TTAGACA2601.14283466E-725.0960454
TATATAC3251.4824764E-924.5383553
TCCTATG7350.023.6755162
Produced by FastQC (version 0.11.5)