FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1981_d12_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1981_d12_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATGGAGAGTTACACCCCAACAAGCTGGAGAATCCAAAAGAAAAGGAT15.0No Hit
GGGGAACTCTGTGCGCACAGGAGGCATCGTCACACGGAGTGAGCCTGCGC15.0No Hit
GCCGAAGGCAGACGCCCAACAACTGAGCCACCCGAGAATGTGGCTCTTAG15.0No Hit
GTGGGTGCGTTGTCCGGGGGCAGCTCGTGTGGGCTGTGGGGTCGCGGGGG15.0No Hit
GGTTGGAAAGGGAACGGCGGTGCTGGCTTCGCCCCCTCCGGGGTGGGTCG15.0No Hit
CCTTCATTTTGAGAAATAGTTTCATTGGTTATTCTGGGTTGATGGTTTTT15.0No Hit
GAGTAAGGGCGCAGGGGTGGCTCAGTCCCGTAAGCACTGGACCCTTCACT15.0No Hit
GGTATCTTCTTCAGGAAAAAAAAACAAAGAGTCTATCTCTTCTTTGCCCA15.0No Hit
AGATCAGAGTGAGGACTGTGGATTTACTGCAACCCAGCAAGGCAGCTGGG15.0No Hit
CTCTATAGCGTATGCTTGGATGATTTTCTACCACATGTGTTTTCTTTGTA15.0No Hit
GGCAGGTTCCTTGTGCTCGGCACGGACCCATCTCTGCACTTTGTCCACTG15.0No Hit
CCCTTTGCCTATGCTTACTTATTATTTCAGCTTCCCATTCTGGATAACTG15.0No Hit
TTATTCAGCCATAAAAAGGAAATTCTGACTCATGGTACAAACAGATGAAT15.0No Hit
GTGCCTGGCTTAGCATAGAGGAGGAGACGTCGAGTTGGTGGTGAGTAGCA15.0No Hit
CGTTCTGGGGCCGCGGGGGGGGTGCGGGTGCGGGTGGGGGGGTGGGGTGG15.0No Hit
CTCTGATGGAGGTACAGGGGGTTTTGGCTCTCGGCAAGGTCCTGTCCCTG15.0No Hit
GTATGCGGATGTCGTGCCCTCTAGGCGATGGGCTATGGATGCCCAGCGAG15.0No Hit
GGATTCAGATGCAGGGAGTACTTATGGGAAGTACTCCCAGGAGAGATTCA15.0No Hit
GAGTAAGCATCTCGGCATGTGAGCAGGGTTGGTAGGTGTGTGTGTGTAGG15.0No Hit
GGCTTGTAAAGTCACCGTTTCTCTGAAGCTGCTGAAATCCTCATGGAGGA15.0No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)