FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1981_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1981_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624967
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2100.065.610731
GCGAAAG2150.064.0746462
CGAAAGC3250.042.3878443
CTTAACG1102.431735E-432.962331
GTATAAG1504.3890243E-529.006851
GGACCGT1000.00607285328.9998876
ATCGAGG1000.00607285328.9998879
TTAACGA1306.477324E-427.8867382
GTACTAG1100.00965773926.3698641
CTAGGGT2252.6479287E-522.5572724
GTGTACA1952.6090498E-422.3129621
CTTTTCG1700.003091581621.323446145
TATATAG2053.662433E-421.2211272
GTCCTAT5850.021.0733551
CATATTG3809.0185495E-920.988443
TACTAGG1750.00365560420.7158622
CTATAAT1800.00429941220.1436441
AACGACT1800.00430339720.140424
ATATAGT1800.00430539120.1388116
GTTATAT2608.056495E-519.5238421
Produced by FastQC (version 0.11.5)