FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1981_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1981_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784682
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9820.12514623758414237No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGAAT855.3405063E-542.646965145
TATACTG2450.041.428485
TCTATAC6500.040.1563153
GTATATA2650.035.572761
TATACCA7750.035.548315
GTTCTAT8600.032.884121
CTATACC8650.031.8496424
GCGAAAG3250.031.2326892
AGCGAAA3300.030.7633881
CGAAAGC3300.030.7594663
TTCTATA9450.029.9224762
GAGTATA8050.025.217337145
GTCCTAT8950.025.1164781
ATACCAC10200.024.8773966
TACCACT10650.023.8262397
GTAATAA3854.2564352E-1022.6016751
GTATTAC2303.1363306E-522.0693871
GTAATAT2303.1363306E-522.0693871
GTAGAAA4058.021743E-1021.4855421
GTAATCC1700.003092246821.3234838
Produced by FastQC (version 0.11.5)