FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1981_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1981_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences716835
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGT950.00472647830.526238
GTATAAT3100.030.4116231
GGTCCGC1000.006073411628.9999168
AGCGAAA3255.0931703E-1126.7766231
TAGTACC2507.824565E-826.0999224
ACACGAC1409.991991E-425.892782145
GTGATCG1409.991991E-425.8927829
TATACTG3751.0913936E-1125.1332595
GCGAAAG3551.546141E-1024.5086822
TACACGT1801.5197296E-424.1665974
GTCGGGG2751.9657273E-723.7288592
AGACCGT1851.8299981E-423.5134476
GGACTGT6250.022.0399366
AGCGAAC2003.101913E-421.7499378
GTACAAG3508.702045E-820.7200071
CGAAAGC4201.2660166E-920.7156723
TCAATAC2455.1087787E-520.7156723
GTACAAT2505.9587983E-520.3056071
GTATATA3601.17146556E-720.1444511
ATACACG2556.950394E-519.9032923
Produced by FastQC (version 0.11.5)