FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1981_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1981_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647221
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC7170.11078132508061389No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG6590.10181993476725879No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCGC450.00963761348.3333324
GCTATAT1504.396338E-529.0000021
ACCCTCG1000.00607289429.07
CCTCGCG1255.1518565E-429.09
CCGTTAC1255.1518565E-429.03
CAGCGGA1358.0796145E-426.8518529
GTATAAG2651.3747558E-724.622641
TAGGAGT2151.8644187E-523.604654
AGGTCGG4002.7284841E-1123.56258
CGTTACT1550.001807803223.3870964
GTATATA2202.2272317E-523.0681841
GTTGACA6300.023.0158731
CTATATA1902.1920081E-422.8947351
GGGTACT1600.002174326522.656251
CCCTCGC1650.002599878521.9696968
TATATAC2353.7065765E-521.5957453
GTATTAA7000.020.7142871
CAGGTCG4201.2660166E-920.7142857
TATAGGC2155.0534867E-420.2325573
GTACCAT1800.004305457720.138891
Produced by FastQC (version 0.11.5)