FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1981-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1981-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784873
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10130.129065466642374No Hit
GTTATGTGTTGGCCTTTGGCTCAAGTCATGAATTTAGGGTCCTGGAATTG10060.1281736026083201No Hit
GTATTATTTTTTCATTTACCTCAAAGTATTTCTGTGTTCCTATTTTGGTT8830.11250227743851553No Hit
ACATTCTACTGAACAACCAATTGGGTTCGTGGGCAAACCAAAATAGGAAC8670.11046373107496373No Hit
TTACCCACCCAGCATGGAGCAACTAAATATGTAGAGCATATATGAACAAA8640.11008150363179775No Hit
GTGTGGGGTTGAAACTCAAAACCACAAGATCAGGAGTCACAGAGCTCCAC7960.10141768158670257No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGC100.007090639144.999568
GTTACGT1450.065.020511
GTACTAG606.791517E-660.435731
TTACGTG2000.047.1278572
GCTACTA1201.9727668E-742.291549
CAAGCGG700.001056186741.4310843
CCCTACG800.002036379236.249897
AACTTCG800.002036379236.249897
GTGATCG800.002036379236.249899
TACGTGT2850.035.6161963
CGTGTTG2650.035.565935
CGACCCG850.002741668634.117542145
TAGTCGG1501.1335051E-633.833238
TATACTG3550.032.6759535
AAGCGGC900.003627814432.2221224
ACGTGTT3200.031.718654
GTGCGAA1601.8764804E-631.718654
TTGATTA4900.031.071335145
GTTGACA7800.030.6827531
GCGATTA1204.057332E-430.20824145
Produced by FastQC (version 0.11.5)