FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1981-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1981-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524262
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC7130.1360007019391068No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG6110.11654478104459219No Hit
GTAGAGGGATGACCTCATGGTCCAGGTGACACACCTGCAGGGAGGAGACA5720.10910575246727781No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC5570.1062445876298492No Hit
GTCTGATATTCCTCTACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCG5560.10605384330735394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG2950.049.1524165
GTAATAT2100.048.3424261
TAATATA2850.038.1614342
GTATAGT1501.1308603E-633.83971
TATAGAT1102.433251E-432.9576032
ATAGTTC1153.158344E-431.5246643
GTAGGCT2807.2759576E-1231.0713446
TATAGGT950.004725326330.5262374
GGATTAG2202.2557288E-829.664671
TAGGCTC2951.4551915E-1129.491457
AGCGAAA2252.8065188E-829.0054571
GTTATAC1000.006069106529.002693
TTATACT1753.7701866E-628.9999244
GTAGAAA2853.2196112E-1027.9850522
GGGATAC3900.027.884542145
GTTGACA3950.027.5368251
CTGTAGG3204.1836756E-1127.187434
ATACTGT4550.027.0878416
GCATTAT1100.00965860726.3685971
ATTGTCA1100.00966759326.3635675
Produced by FastQC (version 0.11.5)