FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1981-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1981-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739125
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC26190.35433789954337896No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG16290.2203957382039574No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA15660.2118721461187215No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA13340.18048368002705903No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA11440.1547776086588872No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC10480.14178927786233722No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA10470.14165398274987315No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT10150.13732453915102316No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG8780.11878910874344666No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA8600.11635379671909353No Hit
GCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAG8510.11513614070691697No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG8440.11418907491966851No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC8360.11310671401995602No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA8150.11026551665821072No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC7730.10458312193472011No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT7620.10309487569761541No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA7610.10295958058515137No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC7610.10295958058515137No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT7580.10255369524775916No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT7420.10038897344833418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG5750.051.6954575
TTATACT5850.050.8152124
CCTTATA6600.046.1424372
TATAGCG3700.045.0734982
AGTAGAA3550.044.9415741
GTCCGTT657.3354697E-444.61522145
GGATAGC2800.044.0176935
TATACTA1851.8189894E-1243.113782
CCCTTAT7300.042.716881
GCGGTAT4150.040.1805766
CGGTATC4400.037.8975877
GTTATAG1001.3889158E-436.2596741
GACAGTA3250.035.6921737
TACTGGA8200.035.3657237
GGTACTG1854.1800376E-935.270145
ATAGTCG1051.8532897E-434.523688
CTTAGAC2551.8189894E-1234.1198273
TTTGGAC4500.033.8354953
GTATAGG11600.032.5086751
GTATCCC5150.032.378529
Produced by FastQC (version 0.11.5)