FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1981-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1981-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences708306
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC300.001934461872.509883
GCGAAAG2700.040.2832682
TCGTACA750.001482614838.669214
TAGTACC2257.912604E-1032.224344
GTCCTAT3400.031.9964311
ATAGTAC2901.2732926E-1130.0040913
CTATAGT1255.1418506E-429.01011
ATGCGCA2502.4447218E-928.999859145
TACCGTA1504.3970635E-528.9998598
CGAAAGC3850.028.2506033
AGCGAAA3900.027.8943231
ACCGTAA1606.825596E-527.1873689
TTAGACT3408.913048E-1125.5899184
TACCTAT1701.03091064E-425.5881127
GTCCTAG5400.025.5181431
GATATAT2859.848009E-925.4474541
GACAGTA3501.2914825E-1024.8570217
TAGGCTG4151.8189894E-1224.4577145
GTTCTAT6750.023.6378571
TACTGAC4353.6379788E-1223.3365152
Produced by FastQC (version 0.11.5)