FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1981-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1981-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences508213
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGT750.001481344938.673862
TATACTA1652.383582E-630.757245
AGCGAAA2252.8008799E-829.01111
GTATATA1753.7585905E-629.01111
CGCTAAG1050.00769795327.6241842
CTATACT2456.419941E-826.6349834
GGTTATA1409.965522E-425.902771
GAATAAC2559.432006E-825.5980321
CGAAAGC2601.140379E-725.100823
GTATTAT3851.6370905E-1124.4898911
GTAGAAA5400.024.1759171
GCGAAAG2751.9611252E-723.7316842
GCCCTAC1550.001803249923.3960511
GCTGTAC1550.001805316523.3914453
GTAAGCC2802.3337088E-723.3079053
TACTTAG2502.2757667E-623.1997477
GTACTAA2202.219433E-523.0770131
GAATATC1952.6055326E-422.3162331
CCTATAC2603.1938653E-622.3118423
GTGTACG1700.003083870521.3316941
Produced by FastQC (version 0.11.5)