FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1980-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1980-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552790
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC6150.11125382152354421No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT752.5464118E-548.3419271
TTACGTG951.0277654E-438.161232
TAGTACC2154.8567017E-1033.7208259
CCGTAGC900.00362689332.222122145
ATAGTGC1153.158665E-431.524493
GTCTTCG950.004721377530.5317441
GTGATCG1703.0085776E-629.8528489
GGACCGT1306.4793724E-427.884536
CCTATAT1050.007698833527.6239591
TATATAA1907.1430095E-626.712862
GTATTAT4950.026.3683241
CGGCCCG1100.00966806326.3635529
TACGTGT1409.983828E-425.8951193
TAGGACT3751.0913936E-1125.1355274
CTACTGA5900.024.5761936
GTATATA2101.5512962E-524.1709631
TATAATA3301.7662387E-924.1687772
ACATAGT1801.5191176E-424.1665946
GTGTATA3402.4938345E-923.4600521
GAATAGT3452.9576768E-923.115876
Produced by FastQC (version 0.11.5)