FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1980-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1980-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066800
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT21930.20556805399325082No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC17820.1670416197975253No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA13890.13020247469066368No Hit
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC12520.11736032995875517No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC12370.11595425571803523No Hit
TTATAATACAGCCCTGATAATGACAGCTGACCATTTTCGGGTTCATGTAC11720.1098612673415823No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC11650.10920509936257966No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG11220.10517435320584927No Hit
AAGCTGTGATGTGCAAGTGTTTTCTCTCGAGTGAATAAACATTGAGTGAC11030.10339332583427072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTTA1109.960786E-846.13596145
GTAATAT3400.038.4054221
TTAGGTA4550.036.6531834
AGCGAAA5000.033.370041
TATACTG4150.033.194045
ATTAGGT5600.028.4872343
GGATTAG7250.028.0168271
GATTAGG6050.027.5694962
TAATATA5400.026.8591692
CTAGTAC1358.074633E-426.8566513
CCAACGC1100.00966565326.3671134
ATCGAAT1100.00967228126.363405145
GCGAAAG6450.025.8682481
GTATTAT6800.025.6036131
GTAGAAA5100.025.6036131
GTGTACA5300.024.6374381
TATTATC5600.024.604922
CGAAAGC6900.024.1732542
GCACCGT14200.023.485716
AGCACCG15050.023.1238045
Produced by FastQC (version 0.11.5)