FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1980-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1980-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789391
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG83091.0525835739196419No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCC78961.0002647610626418No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG78720.9972244426399591No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA73860.9356579945806324No Hit
GGATACACCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGG69860.8849860208692525No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGATCCAAA66850.846855360651439No Hit
ATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAA45020.5703130641215823No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT40630.5147005729733428No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA40110.5081132163908634No Hit
TTCTTAATCCTTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCT39270.49747210191147356No Hit
ATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAGTG34660.43907265220910807No Hit
ATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGC30760.3896674778405125No Hit
CTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCA29030.36775184921034065No Hit
CTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCA28210.35736409459950774No Hit
ATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACACCATG27700.35090341795130675No Hit
GCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTT26970.3416557827489799No Hit
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC22020.27894921528114713No Hit
GCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAG21920.2776824159383626No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCC21610.27375533797573065No Hit
GCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATC20200.2558934672424692No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG20020.2536132284254571No Hit
ATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATATGGGGAA19940.2525997889512295No Hit
CTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGATCCAAATG19530.24740591164581302No Hit
ATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTG19000.2406918751290552No Hit
TTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACA18770.23777823664065081No Hit
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA18360.23258435933523436No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAT17920.22701044222698258No Hit
AAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAG17860.22625036262131187No Hit
TAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAA17720.22447684354141356No Hit
CCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCTAAG16840.21332900932490997No Hit
GATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGA16730.21193553004784701No Hit
GAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATG16630.21066873070506253No Hit
GGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATAT16280.20623493300531678No Hit
ATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAATATGCA15940.20192781523984946No Hit
TCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAA15620.19787405734293906No Hit
AAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATGGCCTCCATCAT15480.19610053826304075No Hit
ATATTCAGAAAAAGGGAGGTGGACAACAAACACAGAGACCGGAGCACCCC15310.19394697938030708No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT15120.19154006062901655No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTTGCCG15040.19052662115478897No Hit
GTACTGATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGA14940.18925982181200446No Hit
TTTCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATA14870.1883730622720553No Hit
AAATAGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTT14820.18773966260066305No Hit
GCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGGTTGTCCCT14640.18545942378365093No Hit
CATCAATATTCAGAAAAAGGGAGGTGGACAACAAACACAGAGACCGGAGC14610.18507938398081558No Hit
GATATGAACTGCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGG14500.18368590470375262No Hit
GGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAA14490.1835592247694742No Hit
GTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGATTTGCAGATGC14340.18165902575529744No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATGTCGCA14310.18127898595246209No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC14310.18127898595246209No Hit
CCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCCAGGTTCCAGG14260.18064558628106983No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATT14240.18039222641251296No Hit
CTGCAATATGCACACACTTAGAAGTCTGCTTCATGTATTCAGACTTCCAT14180.17963214680684222No Hit
CAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTG13980.17709854812127324No Hit
GAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA13860.17557838890993183No Hit
CCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACAG13720.17380486983003354No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG13560.17177799088157833No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC13330.168864352393174No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA13160.16671079351044032No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGT13160.16671079351044032No Hit
GCTTCATGTATTCAGACTTCCATTTCATTGATCCAAATGCATTGTTGAAG13150.16658411357616187No Hit
TTCCAGACTCGAAGTCAATCCGTGCATCAATTCGGGCCCTAGACACCATG13060.16544399416765582No Hit
CAACAGAACACATCAATATTCAGAAAAAGGGAGGTGGACAACAAACACAG12930.16379715502203598No Hit
GCTAGGGAAGAATTTCTCGAATAGAGTGCAGCACTTCTGGTACATTTGTT12910.16354379515347908No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATTTGATG12860.16291039548208683No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATAGCCCTTC12670.1605034767307963No Hit
GTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGA12590.15949003725656866No Hit
CCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAGGGAAG12490.15822323791378415No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA12420.15733647837383502No Hit
GGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTGAGGA12200.1545495198197091No Hit
GTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAAT12130.15366276027975997No Hit
GTTGAAGCACAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACA12030.15239596093697547No Hit
GATCCAAAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTC11960.1515092013970263No Hit
GTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAG11930.15112916159419096No Hit
AAATGGAAGACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTT11930.15112916159419096No Hit
CCATGGACACAGTCAACAGAACACATCAATATTCAGAAAAAGGGAGGTGG11820.149735682317128No Hit
GTTCCAGGTTGTCCCTAAGTGCTTGAACAATGAGTAGTAGTTTTCTCGAT11380.14416176520887622No Hit
ATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAA11300.1431483257346486No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT11220.142134886260421No Hit
CTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATA11190.14175484645758565No Hit
CAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA11010.13947460764057357No Hit
ACCCTATTGATGGACCATTACCTGAAGACAATGAGCCGAGCGGGTATGAT10960.1388412079691813No Hit
ACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGG10950.13871452803490286No Hit
CTCTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCT10890.13795444842923216No Hit
GTGCAGCACTTCTGGTACATTTGTTCATCCTCAAGAATTCCCCTTTGGCT10830.13719436882356145No Hit
GGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCAT10800.1368143290207261No Hit
GTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCC10740.1360542494150554No Hit
CCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTAAAAGTGCCAGCG10530.13339397079520796No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC10470.13263389118953725No Hit
GAACAGGAACAGGATACACCATGGACACAGTCAACAGAACACATCAATAT10410.13187381158386655No Hit
CCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTG10090.12782005368695615No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCTT10060.1274400138841208No Hit
CCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAACA10020.126933294147007No Hit
CCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCTCAG10000.1266799342784501No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT9960.1261732145413363No Hit
CCCCCATATAGCCATGGAACAGGAACAGGATACACCATGGACACAGTCAA9770.12376629579004574No Hit
GCCTATATTCATCTCCACAACTCGAAGGATTTTCAGCTGAGCAATGGCCT9750.12351293592148885No Hit
CCCCAACTCAACCCTATTGATGGACCATTACCTGAAGACAATGAGCCGAG9740.1233862559872104No Hit
GTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTAT9560.12110601717019828No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG9430.11945917802457845No Hit
GCCACAACTATTTTAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA9340.11831905861607239No Hit
ATGAAGGACAAGCCAAATTCACTTCCCTTGCCGTCCGAGCTCTTCAATGG9330.11819237868179394No Hit
CTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGT9300.11781233887895859No Hit
GTCCAGGCCATTGCTCAGCTGAAAATCCTTCGAGTTGTGGAGATGAATAT9230.11692557933900945No Hit
GGCCTCCATCATGCTGGAAATTCCAACTGGCCTCCGATATGAACTGCTAG9220.116798899404731No Hit
GGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCAACTGGCCTCC9210.11667221947045253No Hit
TGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTT9200.11654553953617408No Hit
ATTCACCACTGTCCAGGCCATTGCTCAGCTGAAAATCCTTCGAGTTGTGG9160.11603881979906029No Hit
GAAAAAGGGAGGTGGACAACAAACACAGAGACCGGAGCACCCCAACTCAA9010.11413862078488354No Hit
CTCGAATAGAGTGCAGCACTTCTGGTACATTTGTTCATCCTCAAGAATTC8940.1132518612449344No Hit
CTTCCATTTCATTGATCCAAATGCATTGTTGAAGCACAAGGTGTGCAGAA8900.11274514150782057No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCA8790.11135166223075763No Hit
CTTCTGGTACATTTGTTCATCCTCAAGAATTCCCCTTTGGCTAGTGTTGA8790.11135166223075763No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC8720.11046490269080848No Hit
AAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTCCATATA8700.1102115428222516No Hit
ATCAGGCACTCCTCAATTGCTCCATATAGCCCTTCAAGATCAAAGGTTCC8620.10919810334802398No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT8590.10881806354518864No Hit
TTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAAGTC8580.10869138361091017No Hit
CAATGAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCT8580.10869138361091017No Hit
GTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGCATTCTTTTCGT8530.10805798393951793No Hit
GAATATAGGCTGTTGAACACAGATTTTGCTAGTAAGGTTCTGCACACCTT8480.10742458426812568No Hit
GTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAAT8450.10704454446529034No Hit
GATCCAAATGCATTGTTGAAGCACAAGGTGTGCAGAACCTTACTAGCAAA8440.10691786453101187No Hit
TCTTAAAAGTGCCAGCGCAAAATGCAATAAGTACCACATTCCCTTATACT8390.10628446485961963No Hit
CTTCCCTTGCCGTCCGAGCTCTTCAATGGTGGAACAGATCTTCAAGATCT8390.10628446485961963No Hit
TGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAGCAAAACCCAGGGATC8290.10501766551683513No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC8280.10489098558255669No Hit
ACTTTGTGCGACAGTGCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAG8260.10463762571399977No Hit
GAGTAGTAGTTTTCTCGATTTGCAGATGCTATTCACCACTGTCCAGGCCA8260.10463762571399977No Hit
GAAGGAATATGGGGAAGACCCGAAAATTGAAACAAATAAGTTCGCTGCAA8240.10438426584544287No Hit
ATCTTCAAGATCTCAGCAAATTCTTCTTTCTTAATCCTTCCAGACTCGAA8060.10210402702843077No Hit
ATGCAATAAGTACCACATTCCCTTATACTGGAGATCCCCCATATAGCCAT8020.10159730729131697No Hit
CATCAATTCGGGCCCTAGACACCATGGCCTCCATCATGCTGGAAATTCCA7980.10109058755420318No Hit
AGCACAAGGTGTGCAGAACCTTACTAGCAAAATCTGTGTTCAACAGCCTA7910.10020382801425402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGTA12150.0112.783632
CGCAATT203.8659264E-4108.748764145
AGTAGAA9650.0108.2332841
GGTACTG13200.0103.262955
CAGGTAC13350.0102.1026843
GGTCAAA2000.0101.543871
GTACTGA15600.087.3763356
GCGAAAG1400.082.861452
AGCAGGT16550.082.8303451
TTTCGTT451.2327291E-680.55464145
ACTGATC18900.075.184338
CTGATCC19050.073.070039
AGGTACT19350.073.065814
AGAAACA14950.069.83644
AAATATA2950.068.817145
GTCAAAT2950.068.817142
TAGAAAC15200.068.687783
AAATAGA3500.068.3866961
AACACTG3900.066.926565
TTTGGAC17150.065.528183
Produced by FastQC (version 0.11.5)