FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1980-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1980-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555765
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC14120.25406421778989324No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA8680.15618111971786636No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA8480.15258247640639477No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG8280.14898383309492322No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAG7480.1345892598490369No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTATTTTAGTGCATGTGTG6770.12181407609331282No Hit
ATATTATTCACCTTGGATGTTATTTTATCAATTGCCTTTTGAGTTGAATC6560.11803550061626766No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT6310.11353719647692821No Hit
CACTTAGAAGTCTGCTTCATGTATTCAGACTTCCATTTCATTGACGAACG5970.10741950284742653No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC5930.1066997741851322No Hit
GCTTCAATCCAATGATTGTCGAGCTTGCGGAAAAGACAATGAAGGAATAT5880.10580011335726432No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC5840.10508038469497No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC750.001481331438.674563
GACAGTA4350.036.6675537
TCCGTTC1051.8523954E-434.5246477
GGTACTG1905.4151315E-934.3429375
TATACTA2750.034.2797242
TAACTAT2551.8189894E-1234.1399771
CTACTAG2800.033.661536
ATATCAC1956.9667294E-933.468373
TATACTG3700.033.311625
TTATACT2950.031.9528034
AGGTACT2558.731149E-1131.2780824
AGGTCGG1402.744044E-531.0721828
CTAGTAG3050.030.902399
GTATAGG6150.030.6704651
GATTAAT1204.0402298E-430.2281041
ACTAGTA3201.8189894E-1229.4538388
CCTTATA3450.029.4262982
GTACTGA2752.1282176E-1029.0007026
TAGAAAC5600.028.4879592
TTTGGAC5350.028.4637553
Produced by FastQC (version 0.11.5)