FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1979_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1979_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519049
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTAAGGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCAC7110.136981286930521No Hit
TCCATTACCTCTACCTGCAGAACAACTTCATAACGGAGCTCCCCGTGGAG6050.11655932291556288No Hit
GTAGAGGGATGACCTCATGGTCCAGGTGACACACCTGCAGGGAGGAGACA5670.10923824147623827No Hit
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC5550.1069263210217147No Hit
GTCTGATATTCCTCTACATGGAGAAGAACCAGCTCGAAGAGGTGCCCTCG5550.1069263210217147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG755.638867E-1177.3326649
GTTACGT406.6477456E-572.527331
CCGTAAT350.003560218562.166281
TTGATCG502.0094254E-457.9995145
TTACGTG700.001054714641.440192
CGAAAGC2151.2732926E-1137.0998543
GCGAAAG2201.6370905E-1136.2601662
GCTATAT850.00273571734.1305051
GTACATA1301.6489957E-533.4741481
TATACTG2455.4569682E-1132.5538755
TTAGAGT2257.894414E-1032.2250484
ATATACC900.003625078132.2250484
AGCGAAA2558.731149E-1131.2862951
TAGTACC950.004725422230.5260529
GTAATAT1702.9992152E-629.8641911
TAGGACT2701.70985E-1029.5396274
TTATACT1255.1478244E-429.002544
GTCCTAT6150.028.3033451
TATAAGA2054.0297346E-728.2951644
TAGAGTG2654.5438355E-927.360895
Produced by FastQC (version 0.11.5)