FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1979_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1979_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731503
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGTGGGTGGGTGATGTATGCCCCACACGAAAAGAATGCTCTTTCC26020.35570599163639793No Hit
GTCCTACATCGTCGAAAGATCATCAGCTGTAAATGGAACGTGTTACCCTG16130.22050490565315523No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA15570.21284943465713743No Hit
CACCTATACCGAGCAAACAAATTTGTACATAAGAAACGACACAACAACAA13190.18031368292406183No Hit
GTTTTAGTACCGACCAATAATAATCAATTCTTCCCTGCAGACCATTGACA11380.15557010702621862No Hit
GGGTAAAAACCGCTCTTTTGAGTCAGCCATCTCATACTCCTGTAGAATGA10430.1425831473008313No Hit
TTCCAACACTGTGTCAAGCTTTCAGGTAGACTGCTTTCTTTGGCATGTCC10380.1418996231047583No Hit
ATATAGCGGTATCCCCTCCCCCTCATGGCTCGGGTCCTCAATGCTTAATT10100.13807188760674938No Hit
ATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGAGG8710.11906991495591952No Hit
CTATTGAAGGACTAGTCTATGGCAACCCTTCTTGTGACCTGCTGTTGGGA8550.11688263752848588No Hit
GCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATCCCCTCATTCTAG8460.11565229397555443No Hit
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG8420.11510547461869602No Hit
GCTCCACACAGAGCATAATGGAATGCTGTGTGCAACAAGCCTGGGACATC8280.11319160686969157No Hit
CTTCAATGGTGGAACAGATCTTCAAGATCTCAGCAAATTCTTCTTTCTTA8090.11059421492461412No Hit
GTTTTTACCCTGTTCAAGACGCCCAATACACAAATAACAGGGGAAAGAGC7680.10498931651681538No Hit
CTATACTACTAGTAGTAACAGCAAGCAATGCAGATAAAATCTGCATCGGC7540.10307544876781093No Hit
GTGTTACCCTGGGAATGTAGAAAACCTAGAGGAACTCAGGACACTTTTTA7530.10293874392859634No Hit
GTACTAAAACCAGGCCAAACATTGCGAGTACGATCCAATGGGAATCTAAT7520.10280203908938172No Hit
CTACTAATAGAGGGCACAGCCTCATTGAGTCCAGGGATGATGATGGGCAT7520.10280203908938172No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT7340.10034135198351887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTA2200.056.030122
CGCAATT752.5499767E-548.333107145
GGTACTG1650.048.3331035
TACTAGT2900.044.9997867
GTACTGA2300.044.1302266
AGTAGAA2900.042.5143281
GTATAGG11350.040.255861
GGATAGC2350.040.1061945
GAGTATA2005.456968E-1239.87481145
TAACTAT1502.5902409E-838.6797071
TATAGCG3650.037.7447052
ACTAGTA3550.036.7603878
TATACTG4550.036.648185
ATAGGTG11950.036.406483
TTATACT4050.035.804754
ATATAAG850.002736978534.129151
GCGGTAT4050.034.0121846
TATAGGT13500.033.8378032
CGCTATA3450.033.6276252
GACAGTA2404.3655746E-1133.2290087
Produced by FastQC (version 0.11.5)