FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1979_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1979_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700481
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAC1656.004848E-835.15651
GTAGCCG2353.45608E-1133.936149
CGAAAGC4350.028.3333073
GCGAAAG4150.027.951782
TAGTACT1306.481034E-427.8845864
GTATACC1658.406921E-526.3673761
TATATAA3900.026.0256162
GGATAGC1701.0308672E-425.5882115
GTCCTAT4600.025.2209661
TACACTA3451.07320375E-1025.217375
GATATAC2601.14216164E-725.099711
GTACTGG3801.2732926E-1124.806151
AGTAGCC3251.4824764E-924.5384358
AGCGAAA4750.024.4245151
GTATATA3052.0294465E-823.7738631
TTATAGT2451.9107374E-623.6734474
TAGTACC2151.8647164E-523.604634
ATTACCC1550.001807961623.3870743
TATACCG1550.001807961623.3870745
TATAGTT2202.2275875E-523.0681575
Produced by FastQC (version 0.11.5)