FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1979_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1979_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502548
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1956.9503585E-933.4746781
GTATAGT1652.3760222E-630.7696531
GTTATAC950.00471601530.5383011
GTATATG2553.0031515E-928.4425371
GTATAGG2553.0031515E-928.4425371
ATATATC1958.736792E-626.0280883
GCGAAAG2559.4396455E-825.5957362
AGCACCG1450.001225633124.9998655
GTACTAA2051.28468655E-524.7658181
GTAGGCT2101.5531354E-524.1665346
TATACTG2151.863422E-523.604525
ATACAGC3253.9801307E-822.3075716
CGAAAGC2953.8629514E-722.1207243
GGTACTG2303.1381453E-522.0650965
TATAAGT1650.002599158821.9695784
GCATTAT2003.0918475E-421.7585431
TATAATA3004.5328125E-721.7563742
ACAGTAT3055.329548E-721.3933268
ATATTGA2053.6532347E-421.2278441
GTACTGA2755.1844963E-621.099191
Produced by FastQC (version 0.11.5)