FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1979-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1979-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences631271
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA1550.074.844521
GCGAAAG1950.059.4917952
TCATACG400.00604854.379223
ACCGGTC400.006049895754.3749128
TCCGCAA907.475882E-540.2809033
ACCGTAC1301.6529408E-533.4641341
TCTATAC3250.033.4641343
GTACTAG1102.4340276E-432.9571041
GTATAAG1601.8745304E-631.721211
CGAAAGC3950.029.3693683
ATATAAG2104.978174E-727.6211933
GTCTATA1100.00966527926.3656831
ACTATAT1701.0301593E-425.590223
GTACTAC2401.596547E-624.1666286
GTATTAG2202.2257831E-523.0699731
GTACACC2552.704497E-622.745064
TACTAGG1600.002173287322.6580092
AATCCGA1600.002174288322.656214145
TACACCA2252.6495745E-522.555525
GAGTATA3955.875336E-1022.02528145
Produced by FastQC (version 0.11.5)