FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1979-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1979-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences791408
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC9860.12458807593554778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC3800.040.068223
GCGAAAG4250.035.827962
AGCGAAA4250.035.827961
GTGATCG1251.26477735E-534.7999089
CTATAGG1050.007702842427.6224652
ATCGAAT1050.00770759427.618977145
GTATAAG1801.5186194E-424.1696571
CTATATA1902.1907696E-422.8975722
GCTATAG1902.1907696E-422.8975721
GCATTAT2252.6481057E-522.5583461
TATACTG2252.6506927E-522.5554965
GTTGACA6450.022.4834021
GCGGACC1650.002600424221.9696399
TCTATAC6400.021.5247423
GTTATAT1700.003090012421.3261681
GTAATAA3157.269173E-720.716851
TATACCA8000.019.9374495
TATATAC3301.1367047E-619.7739243
ACATGGT7700.019.7726765
ATTAGAC2959.55309E-619.6622093
Produced by FastQC (version 0.11.5)