FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1979-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1979-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724192
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG400.00605030554.3747945
GCGAAAG2400.048.339822
AGATCGT1053.8227554E-641.4284136
AGCGAAA2900.037.5102161
CGAAAGC3450.033.6253783
GTATAAG1801.2884811E-732.2310031
AAGATCG1000.006073479528.9998875
ATATAGG1306.470892E-427.8922121
GTTATAC1902.191388E-422.8962333
GAATAAC3554.107278E-922.4709071
ATAGGAC4256.184564E-1122.1779173
GTCCTAT8850.021.3052391
ATAGGTC2755.207563E-621.090838
ATATGGC2505.9717604E-520.2999237
ATAAGAC3259.815867E-720.0782343
GTCTAAT2908.224941E-620.005451
TATACTG4752.728484E-1019.842035
TCCTATG10050.019.4802272
ATCTAAC1900.00587709419.0841461
TATATAC3802.0752486E-719.0801933
Produced by FastQC (version 0.11.5)