FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1979-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1979-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653337
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC7220.11050958387478436No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG6610.10117290158065438No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCGT300.001935453272.4998866
CGATCCG450.009637712548.333256145
ACCGTCC1151.4115903E-744.1303678
AGTAGCG750.001483020638.6666075
GAGCGAA1153.1566832E-431.5289291
GTACAAC1652.3805187E-630.7645911
GACCGTC1000.00607298528.9999527
GTATAGG1658.4011415E-526.369651
CGAAAGC2051.289389E-524.7560583
GCGAAAG2351.3292774E-624.6808132
CTACATC3551.546141E-1024.5070043
GTATAGT1851.8268946E-423.5188771
GAATAAC4353.6379788E-1223.3386541
GTGCTAA7500.023.2052941
TTGTTTA10150.022.8571079
TCAGGCG3554.118192E-922.4647549
AGTGCAG6200.022.2177073
TAGCATG8850.022.118612
GTTGACA6050.021.5751691
CAGTGCA6450.021.3565542
Produced by FastQC (version 0.11.5)