FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1978_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1978_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGGCACAAGAGTTGGCCAGGATGGGCTCCAGGGGGGCCGTGGGAGA20.1394700139470014No Hit
CTTACAGAGAGAGCGAGCAACCCCCAGCCTCACAAACTAACTTTTAGAAA20.1394700139470014No Hit
GTTGTAACCTTTGCTCCTGAGAACTTCAGGGGCTAAGTACGCCGGAGTTC20.1394700139470014No Hit
CTTTGAGGGTGTGGCACCACAGACTTTTAACATCCACTGTTGTTGCTAAA20.1394700139470014No Hit
GGGTAGCAACTACTCCATTCAGCCGCTCAGCTTGCAAGCAGCCACGGACC20.1394700139470014No Hit
GTCCACCTGACATGGGTAATATCGAGGCCAGAGCCAATTCCAGTTACTGC20.1394700139470014No Hit
TCTCTTAACTGTGAGGCTGCCAGCACTCAAGGAAAGGGAAATGGCTACAG20.1394700139470014No Hit
AACTAGAACTGTTTGGGTCTAAGACATCAATTGCTGCAATTAAACCCTGG20.1394700139470014No Hit
GTGATGTGAACAAGAAATCAATATTCTTGGGTTAAGCTACTGAGATGGTG20.1394700139470014No Hit
GTATTCAAATGATTCACCAATTGTGCATAACACCCAGTGCTTATCACAAC20.1394700139470014No Hit
GGTGAGGGTGAGCCATCCTGGCAGAAGGCACACATCAGCTGTGACTCAGG20.1394700139470014No Hit
CCACACAGGGGATGCTCCAGAAATGTGATGTCTTCTCTCTCCTCCACCTC20.1394700139470014No Hit
GAGCAAGACATTGGAAGAGCTAATATCTGCTGTGGGCTGCTCTGGGTCAG20.1394700139470014No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)