FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1978_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1978_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTTCATAGAGCATCATACCCATAAAATGACGCCTGCATGCCTGCGGC20.18248175182481752No Hit
CATCAGAAGTTGGCCAGTCCTGGGTTTGCTCAAGTTCTGTAATTGGAACA20.18248175182481752No Hit
CACTTAGGGTGTGTGAGGTTAATTGTGTAAAAAAAAATTAATTGACTTTT20.18248175182481752No Hit
GTTTATTACACCGTGCTCTGGAGACTAGCTCAGGCAAGCAGCAGCATCAT20.18248175182481752No Hit
ATCCTAAGGAGCACTTCTACTGATTCGAGAATGTGCAGCTGGCATGGTAA20.18248175182481752No Hit
GAATGGGCTGCTGGAGGGGTGGATGTCCTGGAGAGGAGTGGCCCCTTTCG20.18248175182481752No Hit
TCATACAGTTAGTTCCCTTAAGCATCTTGCAAGATGTAAGACACAGCCCC20.18248175182481752No Hit
GTATAGGTCTGATCCCTGTGACTGTGCGTCGATCCTGGTTTGTGGCCAGC20.18248175182481752No Hit
CTACTATCAAATTCAGGCTCAATCAGTACAAACCAAAATGTGAATTCAAG20.18248175182481752No Hit
CCATATTACAAATGGGTGGCTTACCTCACAGACCACTGAGGTTTCTGTAG20.18248175182481752No Hit
ATATAGAATGGCCCGGGGCCAGAGAAACAGTGATAAGATGCCATTGAAAC20.18248175182481752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTCT100.006119964145.08
ATGGACT100.006119964145.04
AAAAAAA400.00694032918.12575-79
Produced by FastQC (version 0.11.5)