FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1978_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1978_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGTGTGGAGGAGACAGAAGTCAGAGAAATTCCAAAAGGCAGAGTAA20.13236267372600927No Hit
GCACGTACCTGAGGACCGAGTCCAGATGTTTCTTTCATGTCTGACTTGAA20.13236267372600927No Hit
GTGCTGGGCCATCCCTGCTGCCGCGGTAGCTCCTCTCGGCCACTGCTTTC20.13236267372600927No Hit
GAGTGAGGGAATATGGAAAAGCCAGAGGCAGGAAGCCTAAGGAGGATTTC20.13236267372600927No Hit
CACGGGGACCGCCGAGGTAAGCAGGAACGGTCGTTAGCCAGGTTCTTGGG20.13236267372600927No Hit
GTGTTCTTTTCAGTTTATAGGTCTGCATCTACTTTAAACAATCTTTACAT20.13236267372600927No Hit
GTATATTCCTTTTGCTGCTTTTGGATGGAGAGTTCTGTGTGTATCTGTCA20.13236267372600927No Hit
GAGTGAGAGAGCACAAGAAAGGGGGAACAGTAGGGAAAGAGAGGGAGAAG20.13236267372600927No Hit
CACCTGCACATTTCACCCTCGAGATCCACGGATTGGCATCTACAAAGAAT20.13236267372600927No Hit
CACACACACACATACACATATTCTTTATCCATTCATTTGTTATTGATATC20.13236267372600927No Hit
GGATTTCACTGTACATATTTCATATACATGCACATACTCCAGCTTTCAAT20.13236267372600927No Hit
GTAGAGAGATTAAATAAGAAATCAAAAACTTCCAACAAGCAAAAGTCCAG20.13236267372600927No Hit
TCTCTACTGGGCACCTCCCTGGCAAAGAGATGGAGGTTCCAGCCCGTGTC20.13236267372600927No Hit
TGCATACACAACAATAAGTTTTAAAAAGGTGCTCAACATCATTCATCATC20.13236267372600927No Hit
GCTTTGAAGTCATTTAGGCTAGCGTTTGAAGGTGGCTCTGCTCTTGATAA20.13236267372600927No Hit
GCTTTATGGAGATTTGCTGAAGTTTTTAAAAAAGATTTTATTTGTCAGAG20.13236267372600927No Hit
GGTGAAGCCAGGTGGGTTCTTCAGGGGAAGACGAGTGGAACGTTCTGGGC20.13236267372600927No Hit
GGTAGAAACAGCCAAGGCAACTGATCAGGAGCTCAGGAGAGAAGTCAGTG20.13236267372600927No Hit
GGCCTATGTTCCCGGATGCTCACTACGTGCACCAGGCCTGAAAGCACTCT20.13236267372600927No Hit
GTTCTGATGTGTTCCTTCTACTAACTTTGGACTTTATTTGTTCTTTTCTG20.13236267372600927No Hit
AGCTCATTCAGTAACGTTGGGCAAGTTGTTCTGTCCTCTTGCCACAGCTT20.13236267372600927No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)