FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1978-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1978-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808225
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3050.054.6951871
GCGAAAG3250.051.3229752
GATATCG450.00963890948.332789
CGAAAGC3450.046.2427943
GTATAGG2200.039.5621341
ATTAGAC750.001481498738.675793
GCTACGG1402.7462453E-531.0710729
GATTAGC1000.006074118428.999668145
GGCTACG1658.41727E-526.3633338
TCCTACG1500.001494125924.1663887
CCATTAC3905.002221E-1022.3143392
GTTAGCC1952.6099713E-422.3129563
TAGACAG3655.673428E-921.8517675
ATAGGAC4006.87578E-1021.7551333
CTATAAC2704.4269073E-621.490541
GTACCTA3406.4283086E-821.3232846
GTATAGT2806.0657094E-620.7230221
GTATAGA2857.069355E-620.359461
CCATCGA2155.055375E-420.2323239
CCTATAT3051.2698236E-519.0244121
Produced by FastQC (version 0.11.5)