FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1978-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1978-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789678
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAA900.003627864932.2220547
TATATAC3750.029.0035213
ATATACC3052.3646862E-1128.524444
CTATATA2507.807648E-826.1064762
AGACGGT2507.8267476E-826.099861145
GCTATAT3253.9713996E-822.3160511
TATACCT4058.058123E-1021.4813695
GCGCTCG1700.003092318821.3234189
GTATTAG2857.071887E-620.3585051
AATACGT1800.004306412320.1387845
CCTAGGT2908.2372335E-620.0024283
GTATATA3651.3538738E-719.8704591
GTATAAT3051.2702767E-519.0235211
TATGGAC4354.2611646E-818.3355583
ACCGTGC2000.00791488118.1249058
GATTATA3252.1949334E-517.8528421
GTAGCAC3252.1997212E-517.848323
TAATACA8550.017.8069254
CAATATT2450.001216660117.7550094
TATACCA11050.017.7148385
Produced by FastQC (version 0.11.5)