FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1978-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1978-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907829
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3250.051.324441
GCGAAAG4400.036.2558362
TGTAGAC2151.8091669E-830.3503823
CGAAAGC5400.029.5385423
GTAATAC2001.0626343E-525.3832821
CAGATCG1450.001226385824.9998959
TATACTA1450.001226385824.9998955
TAGACAT6450.023.6045515
CTATACC5850.021.0694484
TATTAAG4901.8189894E-1120.7176232
GTGTTAC6200.019.885521
ATTATAC1850.0050450419.595593
GTCCTAT7050.019.5453851
TCCTATG9050.019.2296162
AGTAGTG8750.019.0570645
TTATACT2701.0786088E-418.7972534
CATATTC8500.018.7646279
GTATTAT5056.166374E-1018.6694581
GATATAT3953.1111995E-718.3604221
TATTATC5209.131327E-1018.127922
Produced by FastQC (version 0.11.5)