FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1978-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1978-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653640
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA350.003566003462.142628145
GCGAAAG1900.049.6126672
AACGGCG450.00963779448.3331577
ATTATAC1807.2759576E-1140.2807123
ACAGCCG3100.032.7418149
AACCGCG1204.056611E-430.2082217
CCGTAGA1000.00606397229.0087721
GTAGTGA2252.8139766E-828.9998936
AGCGAAA3550.028.6001951
GCGGTCT1606.824751E-527.187399145
TATTATA3800.026.7145142
CGAAAGC3607.2759576E-1226.1824633
AAATCGC1958.748679E-626.0255478
CCGTTCT1450.001226015324.9999078
GTGTTAT3301.7644197E-924.1739751
CAACCGG3601.8371793E-1024.166578145
CTATTAT2151.8600966E-523.611791
AGTAGGC3702.6011548E-1023.5152263
TATACTA1550.001807860323.387015
GTACATA3452.9504008E-923.122931
Produced by FastQC (version 0.11.5)