FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1977_d04_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1977_d04_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1387
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAAAGAACTAAGAAATTTGATGTCGCAATCTCGCACTCGCGAGATA30.21629416005767843No Hit
CATCTAGGCAGAGGACAGTTTAAACAGAAGCAAATTGGCATCTGGGTCCA20.14419610670511895No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG20.14419610670511895No Hit
GTAGCATTTGGTTTGCAGATACTTGGGTGAGGATTTTTATGTTTATGTTC20.14419610670511895No Hit
GACCAGGATTTCCCATGAATATGAATGCCTCCCTCTTCACTAGGAGACCG20.14419610670511895No Hit
ACTTGATACCTCAAGGAAACAGAAAAGAACAAATAAAGTCCAAAGTTAGT20.14419610670511895No Hit
AGATTATATAGGCATAAAGAATCTCCAGGAGCTCAAACAAGAGATGGATA20.14419610670511895No Hit
CACATAACCCCGGGGGGGCAGGGGGAGGCCACGGCAGATCAGGAAGTCAC20.14419610670511895No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA20.14419610670511895No Hit
GGTGAGGGTGAGCCATCCTGGCAGAAGGCACACATCAGCTGTGACTCAGG20.14419610670511895No Hit
GTCTGAAAACAACTGAATTGTGGGATCTCTAAGGACCCTTCCAGATGGAC20.14419610670511895No Hit
AAGAAAAAGAATCCATGGCACCAAAACAGGCTCAAGGAGAAACGTGAATG20.14419610670511895No Hit
CTGCACGGCAGGCCCCTTCCTACCACTAGGCCGAGAGGGGCTGCATCCAG20.14419610670511895No Hit
GAGGAAGGCTGGGTGTGGGCCTGAGTTTGGGTCTGGGCCTGGGCCTGGGT20.14419610670511895No Hit
GAATCTGGGCTTGAAGGGTGGATTTCAAATCATTTTTAACAACACGGCAC20.14419610670511895No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)