FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1977-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1977-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800579
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAT1956.9303496E-933.4904631
CTATCGT950.004726988330.5259889
GTAGTAT6200.024.5776771
CTAATAC3052.0281732E-823.7761753
GTATATA1902.179845E-422.9145261
GCGTGCG4154.3655746E-1122.7105989
TGTACTC2303.1412732E-522.064985
TATAACC2003.101205E-421.7511254
GTATTAT4701.0913936E-1121.6144121
TGTAATA3406.402661E-821.3312932
TAATACT2755.2061914E-621.0924
TATACTG3809.036739E-920.9866165
GTCCTAT7000.020.7321911
GTGCGAA1750.0036583320.7140647
GGGTAGC2857.0422175E-620.368471
TATTACC2155.0425314E-420.2399252
TCTATAC9700.020.1852383
TCTAATA3601.1708107E-720.1462212
GTATTAG2556.909206E-519.9191651
GTATTAA4402.237357E-919.7898181
Produced by FastQC (version 0.11.5)