FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1977-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1977-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833661
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC13260.15905745860727563No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10200.12235189123636586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1450.070.029021
TTACGTG1900.053.4335822
GTGATCG1303.6975325E-739.038259
CGTGTTG3200.036.2498055
TACGTGT3000.033.835183
GCTATAT1352.135714E-532.2355841
ACGTGTT3150.032.2239844
TATTCCG900.00362798332.222056
GTTGACA7750.031.8196411
GGATAGC2601.10958354E-1030.672915
GACGCTC1204.057601E-430.208176
ACATGGT9300.027.28485
GGTACTG3357.4578566E-1125.9700095
TCGCCAT1409.993508E-425.892717145
ATTATAC2001.0647405E-525.3763853
GTACTGA4350.024.9998656
GTATTAG2051.2857912E-524.7663611
GCGAAAG3551.546141E-1024.512792
CTAATAC2101.5540081E-524.1679863
CCGTATG1550.001808294123.3869729
Produced by FastQC (version 0.11.5)