FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1977-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1977-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1155887
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATTCACTTCCCT14830.12829973864227212No Hit
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG11950.10338380827883695No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA11770.10182656263112225No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1402.7102942E-1046.6306841
TATACTG9000.040.280695
TTACGTG1854.1691237E-935.2819752
TTATACT8500.030.7134134
AAGGTCG2651.382432E-1030.0939089
CTACTGA10600.029.4099566
GTTATGT6700.029.2311741
TACTGAA11100.028.7383277
GTAACGG2156.099017E-726.9903681
GGTCGTT2156.1267383E-726.9763587
ATACAGC8200.025.639886
TAGGACT5800.025.006134
TATAATA9500.024.4291572
GTAGAAA8350.024.3194472
ATACTGG11350.024.2727816
AGCGAAA4800.024.1788711
AACCGCG1500.001494538724.1663197
GTATTAT10300.023.9441261
ATAAGAC2502.2748754E-623.2056923
GTTGACA9800.022.9452551
Produced by FastQC (version 0.11.5)