FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1976-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1976-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924564
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGACCCATGCCCTGCTCCCCTGAAAAGGGCCAGGGACACAGAGTGC15590.16862001981474511No Hit
ATTTTATATTGTCCCTCTGGTTCACGGATCTCTGCACTTGTTTTCTGAAG13550.14655556565040387No Hit
GAATAGAGAAGAAAGCTACCGCTGAGGTTGCGCGTGAGCTTGGCCTGCTT9490.10264297549980314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3100.077.2186051
TAACGGC450.00963919348.332976
AACTACG450.00963919348.332977
GCGAAAG5300.045.1533852
GCGTACC1351.02591E-842.9626439
CGAAAGC6150.040.0875053
GCGCGTA1951.70985E-1037.1792077
GTCTAGG2950.036.8840831
CGCGTAC1656.0163075E-835.1512538
GTATACG1458.6612454E-735.0186771
GTACTAG1905.3969416E-934.360431
GGCGCGT2601.10958354E-1030.672856
GTATAGG1306.462384E-427.8994961
TACGTGC1855.8134046E-627.4337124
TGTGCCG1907.155084E-626.710327145
TACTAGA2756.7448127E-926.370572
GACGGGA1409.978695E-425.8996662
TAGTACT3108.58563E-1025.7270054
TATTGTC8800.024.7157277
TATATAC2351.3284644E-624.6846713
Produced by FastQC (version 0.11.5)