FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1976-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1976-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147231
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC5400.051.0270733
AGCGAAA5500.050.1102371
TAGGCGT657.3325494E-444.6209264
ATAGCGG1557.403287E-1042.098335
GCGAAAG6650.041.437332
TAGCGGT2008.953066E-932.6247866
ACCGTTC1504.3994623E-528.999818
CTATACT6650.028.3493824
TATACTG8750.028.172475
CCTATAC5800.027.5046123
TATATAG2654.5583874E-927.3595035
GTATAGG2507.8036464E-826.1100711
GTATAGA2601.1407792E-725.1058351
ATAGGTC1751.2544537E-424.8613113
TATACCT7350.024.659702145
GGGTAGC7650.024.650031
TGTACCG7450.024.329765
GAATAAC1801.516502E-424.1759911
TACTCGC900.005164961324.1717762
GTATTAA5450.023.9541911
Produced by FastQC (version 0.11.5)