FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1976-d04-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1976-d04-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1087107
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA12930.11893953401091154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC3350.051.944813
GCGAAAG4500.038.670032
TATATCG800.002036743636.2498175
TATACTG8100.034.907235
CGCTATA2653.6379788E-1232.8330422
GTATAAC1551.4631169E-632.747791
TATACCG2950.031.9489925
TATAGCG3200.031.7215062
CTATACT4700.030.8523274
TTATACT7400.028.4133244
ACTACCG1555.5010474E-528.064373145
ATACCGA2853.237801E-1027.9823136
TTAAGCG1409.992766E-425.8939174
TACCGAG3151.035005E-925.3173317
CCTTATA7550.024.9690572
AGCGAAA7000.024.8615911
CTCTATA3601.8371793E-1024.1687682
CTAATAG6350.023.9773584
GTCGGTA3554.125468E-922.464676145
CCTATAC5300.021.8886953
Produced by FastQC (version 0.11.5)