FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1976-d02-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1976-d02-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences896111
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGTAGAGGTCTTATTTTCTCAATTTTCTTTCTCGTCGATTCGTTG10990.12264105674408639No Hit
CTCTAGCAGCGTGTGCCCCAGAATCACACCCCAGGGCCTCCGCTTGCTGG9890.11036579173785391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTGT3300.057.1200038
GGGCGAT651.0936896E-555.7680479
CGAAAGC3600.052.365853
GTATTAG701.6926973E-551.801961
TATACTG2450.047.3459365
GCGAAAG4050.046.547422
TTATACT2600.044.6144374
TATCTCG700.001056692241.4276969
TACGAGT1451.927765E-839.999157145
GCGTGTG4950.038.0799989
TCGGTCC1153.1622316E-431.5210748
AGCGAAA6050.031.1668011
TATCAGG3850.030.1325932
GTCTAAA1753.7648333E-629.0090981
GTATAAT2054.027297E-728.301561
CTAGCAG7250.027.0024413
CCGTTCA4350.026.6661019
TATAACA1658.4188236E-526.363084
GACGGTG2559.485848E-825.5876947
TAGAACC1751.255764E-424.8566174
Produced by FastQC (version 0.11.5)