FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1975_d08_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1975_d08_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length151
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGATCTTTGATCCATGGACAATGATTGTCTGGGTCTGGAAGAACTGC14.761904761904762No Hit
ACTACTGACATTGTTTTACATTTTAATGTCTGGTTTGGTAAAAGATGGTG14.761904761904762No Hit
ACCTAGGATGGCAGGGTTCAAACTTGTCTGAAATGTTAAGGGCAAATCTG14.761904761904762No Hit
GGCTGTTGACGCTTGGCCTCCCGCCCCGCCCTAGTGTTTCTCTCGAAGGA14.761904761904762No Hit
GCCCCAAACCACACGAGGCCTCACAGGAGGAGGATTCCAGGAGTGCGTGG14.761904761904762No Hit
GCTTTGGGCGTTCGCTCTGTCGCCTCAGGGAAGAAGAGCCTCGGGAGAGC14.761904761904762No Hit
GCTCATTCAGTAACGTTGGGCAAGTTGTGCTGTCCTCTTGCCACAGCTTA14.761904761904762No Hit
GAATTCAACATAACTCAGGTCAGTTTAAATAACATCCTTTTGCAAAGTGT14.761904761904762No Hit
CCACTACAGTTGGGGGTCAGCTGGTCGGGTGCTGCGACCGTTTTGTGTGA14.761904761904762No Hit
GCTGAGGTACCCAGAGGCCCCGCAATCCCCCCAGAAAGTGCCTGGCTGGG14.761904761904762No Hit
CCGCGGGCCGGTGGCAAGCGTCACCCGCCTCCTGTGCGAAGAGACGGAAG14.761904761904762No Hit
CTCCAGACCTGGCCTCAGGGCCACATCCTAAACCAGAAAATGGCCAAATC14.761904761904762No Hit
TCCCTGACCTGCCCGCACTGGCTGTTCGCTCCGTGCTCCTTCACACCGTA14.761904761904762No Hit
GTCTCAATTCTGTTTTAATTTAGCTGTCATGCAAGTCTGCAGGAGCCCAA14.761904761904762No Hit
TAGGAAAGGATGCCCTACTTGCACACTGGACGCAGAGCCACCCTCAAATT14.761904761904762No Hit
CGGGTAGGCGGGTGCGTGGGGACGCGCGTGTGCCGAACTGTGCGCCGGTG14.761904761904762No Hit
GTCCTGAGGTCAACTGCACCACATCATCAATAGCACACTTGGGGGAGGGG14.761904761904762No Hit
CTGTTCAGGAGGAGACAAGGGAGGAGGAGGAGAGAAGGAAGAAGCAGGAA14.761904761904762No Hit
ACCAAGCTCACACGCAGGAAACATGCTTGACACCACACGACACGACAAAG14.761904761904762No Hit
CTACTATGTCCTGTGCCTGTGCTTTTGTTATGTTAAATTTGAGGCGAAGA14.761904761904762No Hit
GGTGTGTCCAAGGGGGGACAGAGCGTCCCCGGTCGCAGAGGCGGGCGGAT14.761904761904762No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)