FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1975_d06_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1975_d06_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGAGTGGGTGAGGGGGAAACAGGCTTCCCAGCAGAGCAGGAAGCCCA14.0No Hit
CCTTGGAGCCTACCTCCCCTAACCTGCTAATCTCTGGGGGGAAAAGGAAA14.0No Hit
ACTGATAATGGTATTAATGCAGTAAAATGATGCAACATTGAGAAATATTT14.0No Hit
GAATGATGGGGGGTGTAGCCTGCGGTCGGCAAGGTGCGTGCGGGGGCCAT14.0No Hit
GGTTACTGTGGGCTTCAGAAGGGAGTGTGAATACAAAGCATCTTCAGAAA14.0No Hit
TGGCACACTGTCAGCACAGTGCATCAAGCCTTCCTCCCAAGTGGTCATGA14.0No Hit
CAGAGAGTGCACAAGCATATGCTAGAGAAGGGAGGGTCAGAGGGAGAGAG14.0No Hit
GGTTATGTGACACCGGGGAAGATGGTGTCTCTCCACTGGCACGTTCCATT14.0No Hit
CTTCTCTTGTGTCAAAAAGCAGCCAATATAAAAGATCTGATGCCTAGAGT14.0No Hit
CAACAATGCTGTCAAGTTTGTATTATCTTCTTGGGTCCAAGATCTCACAC14.0No Hit
TCTGAGGCGCCCCCGGAACATAATCTCCGAGCCCCCAAACACAGAGGAGG14.0No Hit
GCATTTGAATCCCTGACATTTGTGATATAATAAGAGTAGTTACCACTAGT14.0No Hit
GTTTAAGAGTGTGGTAATTTGAATGAACAGGGGGAGTCCTTGCCCTGTGA14.0No Hit
GGTTGGCACAGGGGCGAGCTGGGGATGTCCCAGGGCCGCCCACTCGGTCT14.0No Hit
CACCTGCAGGGAGGAGACACAGGAAGTTACTAGCCTGCTGGACCTCTGGA14.0No Hit
AGGCAGATGTCGCCAATCAGTGTAGTTCTACATAAGGACACGACAGATGA14.0No Hit
AGGGTGGAGGTGCCCTAGGTGGGGGGAGCTTTCTGTATGGAGCCGATCGT14.0No Hit
CACAGCCTCACCCTACATGGCGAAATCCAGTAAAACTCCCAGCCACCCGA14.0No Hit
GATCCAGGTCCAACCAAGGACCCTAGAATCGAGCCCTTAGCTGAAGGCAG14.0No Hit
GGGTGGGTGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGG14.0No Hit
CTTTTGTTTATTGCTTACAGAAAGCAAGCTAATGTTAAAAAAGATAAAGA14.0No Hit
GGCTAAGGGTCTTCTCCAGCAAGTCCCAGAGCTCCTGAGTCACAGCTGAT14.0No Hit
TGGGCCTTGTGGGGAGCGGGGAGGGGGGGTAGTTGTGTAGTGTGCGAGGC14.0No Hit
CTCCTGATCTTGTGGTTTTGAGTTTCAACCCCACACTGGGCATAGAGCTC14.0No Hit
TGGGGGGGGCGCGGGTTTCGGGAGTGCAGGTTTCCCGTGTGTCCGTGGGG14.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)