FastQCFastQC Report
Thu 10 Dec 2020
HK7MWAFX2_n02_1975_d02_rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1975_d02_rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTATTGGAAAGGGGGATGAATATAGAAGATCCTGTAGCAGCCATCCC15.555555555555555No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC15.555555555555555No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTATCTTAG15.555555555555555No Hit
CTGTAAGTGGGGAGACCAAGCACTTGAGAGGCTAAGGGTCTTCTCCAGCA15.555555555555555No Hit
GGGTGTAGGCCGGGGCCCCAGAATGTGCATTTCCAGCAGGTCCAGGCGCT15.555555555555555No Hit
CCATAGGCTTCACAGCCCCCCTTTATCTGGATAGACAGTTCAGGAAGGAG15.555555555555555No Hit
TTCAGCGGATCATTCAGCTCACTGGCATTTGTTCCGAGGAAAGGACACGG15.555555555555555No Hit
AGCTCTGGCGGTTCATTTTGAGCTTGGCCTGTGCCCGCGGTCGTACTGCT15.555555555555555No Hit
ATCAAATGCTTTTTCTGCATCTGTTGACATGGTCCTATGATGTTGATCCT15.555555555555555No Hit
CGTAAGAACTCATGGGCTACATGCACAATCGTCTCTTTCTAACTTTGTGT15.555555555555555No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTTTACTTTTCTTA15.555555555555555No Hit
CGGAATGAAGATAAGGTGTAAAGTCTCCTCGGGTCTCCCGGAACAGTTTT15.555555555555555No Hit
CACTCCGACTATACGGTTCTGAAACTTGACAGCCCACACTGCAATACGTA15.555555555555555No Hit
GGCAGGGGGAGGCCACGGCAGATCAGGAAGTCACTCTCAGCAGCTCGACG15.555555555555555No Hit
ACATACAGGTCTCCCTTTAAACCTTCTAAGTGCCCCCCACGGAGTTACAG15.555555555555555No Hit
GTACACATCAGGAAGACAGGAGAAGAATCCCGCCAGCATTAAGCATCAAT15.555555555555555No Hit
CCCTTATACTGGAGATCCCCCATATAGCCATGGAACAGGAACAGGATACA15.555555555555555No Hit
CCCAGGATCAGACCTCATCAGCCACAGGAATCTCGGACTCGACCCCTTTC15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)