FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1975-d12-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1975-d12-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886470
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTATACCACTGTGGTTGGAAAAGATGGTTGATTTGATTTCAGTCCTC11530.13006644330885422No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTATG9000.026.5876882
ACTACGC2207.4810305E-726.3649793
CGATACG1100.009671261526.3634937
GTCCTAT8650.025.9971831
GTATTAG3408.913048E-1125.6025371
CGTACGC1701.0312013E-425.588097145
TATACAT3751.0913936E-1125.1331965
CTACGCT2351.3302179E-624.6807164
ATACCGC5500.023.7271426
AGCGAAA4950.021.9819761
TACACCG2053.6661222E-421.2193975
CGAAAGC4801.4551915E-1121.1492982
GAGCGAA2455.0912044E-520.7258631
GATACCG6300.020.7141725
GGGTACT2505.9737675E-520.299897
CGGGAGT2155.0557E-420.2324494
TAGACAG5750.020.1738035
CTATGAT10400.019.5191254
TATTATA3001.10373E-519.33652
TGATCCG3401.5145015E-619.1910729
Produced by FastQC (version 0.11.5)