FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1975-d08-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1975-d08-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074937
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3600.044.3154221
GCGAAAG3950.040.386862
TATACTG4350.033.3361055
CGAAAGC4950.032.22643
GTGATCG2207.487488E-726.3633739
TATAATA10350.024.5212272
ATACAGC8000.024.4696456
GTATTAG2802.3379289E-723.3087621
GTATAAG2502.2750737E-623.2051681
GTTATGT7800.022.312661
TACAGCC9100.021.5107757
ATCGAGC2455.1148523E-520.714089
ATAATAC13850.020.4178093
ACCGTGT7900.020.1896728
CGCAAGC2556.948686E-519.9054642
AGCGTGT2608.0786485E-519.5190378
TTATAAT11200.019.4239671
GTTATAG2256.85785E-419.3376391
GCGTGCG3051.2751987E-519.0162059
GCACCGT7250.019.0006966
Produced by FastQC (version 0.11.5)