FastQCFastQC Report
Fri 11 Dec 2020
HK7MWAFX2_n02_1975-d06-rep2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHK7MWAFX2_n02_1975-d06-rep2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993105
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCATGTTGTTTAGTCTCTGTGTTTATGAATTTCCAGTTTTCTTCTTG10920.10995816152370595No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT1400.062.1612931
ACGAATT502.0102928E-457.9996766
TTTACGC450.0096316648.34282
CGAATTA657.3367584E-444.615147
TTACGTG2150.040.473042
CGGACCG800.002036663736.2497985
AATCACG850.002739344734.124332
TGCACCG1153.1621865E-431.5215645
CTATATA3005.838956E-1026.5912171
GACCGCA1701.0313247E-425.5880937
ACGTGTT3501.2914825E-1024.8595094
CTACTGA11700.024.7861866
TACTGAA11450.024.6941857
GTATTAT10600.024.6299461
GTTGACA8250.024.613361
TATACTG3301.7735147E-924.1665335
GCGAAAG3753.074092E-1023.2045442
ACATGGT8150.023.1287065
TAAGGTA5050.022.9724834
CGTGTTG3803.6379788E-1022.894615
Produced by FastQC (version 0.11.5)